منابع مشابه
Urease assay and urease-producing species of anaerobes in the bovine rumen and human feces.
A growth medium and test were developed for rapid detection of urease in fermentative anaerobic bacteria. Using nonselective rumen fluid roll-tube agar medium and the new test, it was confirmed that Peptostreptococcus productus is often the most numerous urease-forming species in human feces. Also, some fecal strains of Ruminococcus albus, Clostridium innocuum, and Clostridium beijerinckii prod...
متن کاملStudies on Bovine Rumen Bacterial Urease 1
The propert ies of rumen urease were studied. In the absence of high concentrations of dithiothreitol enzyme activity was rapidly lost; in its presence, the enzyme could be solubilized and purified in high yield. Maximum activity was observed between pH 7 and 8.5. The Km for urea with partially purified enzyme was .004 M with Vmax of 300 to 400 #moles/mg/hr. No divalent metal ion requirement or...
متن کاملGastrointestinal urease in man ' Part I Activity of mucosal urease
The relationship postulated between hepatic coma and hyperammonaemia implicates the gastrointestinal tract as a major source of the excess ammonia in the peripheral blood of some patients with cirrhosis (Butt and Summerskill, 1961; Chalmers, 1960; Sherlock, Summerskill, White, and Phear, 1954). Conventional therapy, comprising protein restriction, antibiotics, and enemas, is directed toward eli...
متن کاملIsolation of Yersinia spp. from bovine feces.
Yersinia spp. were sought in 618 fecal samples from cows. Four strains of Yersinia enterocolitica, serotype O:12,26 (one), O:13,7 (two), and O:18 (one); seven strains of Yersinia kristensenii, serotype O:11,24 (five) and O:12,26 (two); and one strain of Yersinia pseudotuberculosis serotype IIB, were isolated. This is the first time that Y. pseudotuberculosis has been isolated from cows in Japan...
متن کاملUrease activity in microbiologically-induced calcite precipitation.
The role of microbial urease in calcite precipitation was studied utilizing a recombinant Escherichia coli HB101 containing a plasmid, pBU11, that encodes Bacillus pasteurii urease. The calcite precipitation by E. coli HB101 (pBU11) was significant although its precipitation level was not as high as that by B. pasteurii. Addition of low concentrations (5-100 microM) of nickel, the cofactor of u...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1982
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(82)82475-2